Bibliography
90 papers that shaped what is known about Caulobacter, 25 of them marked as landmarks. Titles, journals and years were copied from PubMed and Crossref records, not from memory, and each note says only what the abstract supports.
Cellular Differentiation in Stalked Bacteria Nature 196:1189-1192
Early study of cellular differentiation in stalked bacteria, cited by Shapiro et al. (1971) as one of the foundations of the Caulobacter model.
landmarkOrigins of the modelPolar developmentdoi:10.1038/1961189a0
Biological properties and classification of the Caulobacter group Bacteriol Rev 28:231-295
Classic review describing the biological properties and classification of the caulobacter group of stalked bacteria.
landmarkOrigins of the modelPolar developmentdoi:10.1128/br.28.3.231-295.1964PMID 14220656
Bacterial differentiation Science 173:884-892
Proposed Caulobacter as a model for prokaryotic differentiation, defining in synchronous cultures a life cycle of sequential, polar morphological changes.
landmarkOrigins of the modeldoi:10.1126/science.173.4000.884PMID 5572165
Envelope-associated nucleoid from Caulobacter crescentus stalked and swarmer cells J Bacteriol 132:294-301
Isolated envelope-associated nucleoids from stalked and swarmer cells and introduced a Ludox density-gradient method for preparing large quantities of synchronous cells.
Origins of the modeldoi:10.1128/jb.132.1.294-301.1977PMID 334726
Mutational analysis of developmental control in Caulobacter crescentus Proc Natl Acad Sci U S A 74:124-128
Conditional division mutants showed cell-cycle events form dependent pathways, with flagellin synthesis and stalk formation coupled to specific cell-cycle steps.
Origins of the modelCell-cycle controldoi:10.1073/pnas.74.1.124PMID 264665
The caulobacters: ubiquitous unusual bacteria Microbiol Rev 45:123-179
Broad review of caulobacter biology, framing the group as ubiquitous yet unusual bacteria.
Origins of the modelReviewsdoi:10.1128/mr.45.1.123-179.1981PMID 7012570
Isolation of a Caulobacter gene cluster specifying flagellum production by using nonmotile Tn5 insertion mutants Proc Natl Acad Sci U S A 79:6797-6801
Used nonmotile Tn5 insertion mutants to clone the flaY/E/F/G flagellar gene cluster and showed it contains at least three promoters.
Origins of the modelPolar developmentdoi:10.1073/pnas.79.22.6797PMID 16593248
Cascade regulation of Caulobacter flagellar and chemotaxis genes J Mol Biol 194:71-80
Used the transposon promoter probe Tn5-VB32 to follow flagellar gene transcription, showing flagellar promoters are temporally regulated in the cell cycle.
Polar developmentdoi:10.1016/0022-2836(87)90716-9PMID 3039148
FlbD of Caulobacter crescentus is a homologue of the NtrC (NRI) protein and activates sigma 54-dependent flagellar gene promoters Proc Natl Acad Sci U S A 87:2369-2373
Showed FlbD is an NtrC-like activator that stimulates sigma 54-dependent promoters of late genes in the flagellar regulatory hierarchy.
Polar developmentdoi:10.1073/pnas.87.6.2369PMID 2315326
A temporally controlled sigma-factor is required for polar morphogenesis and normal cell division in Caulobacter Genes Dev 6:2395-2408
Showed sigma 54 (rpoN) is cell cycle-regulated and required for both stalk and flagellum biogenesis and for normal cell division.
Polar developmentDivision & shapedoi:10.1101/gad.6.12a.2395PMID 1459461
Cell-cycle control of a cloned chromosomal origin of replication from Caulobacter crescentus J Mol Biol 226:959-977
Isolated the chromosomal replication origin and showed an origin-based plasmid replicates with temporal control resembling the chromosome.
Replication & segregationdoi:10.1016/0022-2836(92)91045-qPMID 1518064
A Caulobacter DNA methyltransferase that functions only in the predivisional cell J Mol Biol 235:472-485
Identified CcrM, a GANTC adenine methyltransferase expressed only in predivisional cells; constitutive expression caused aberrant cells and extra initiation.
landmarkDNA methylationdoi:10.1006/jmbi.1994.1007PMID 8289276
An essential single domain response regulator required for normal cell division and differentiation in Caulobacter crescentus EMBO J 14:3915-3924
Identified DivK, an essential single-domain response regulator phosphorylated by the PleC and DivJ kinases, required for normal division and differentiation.
Phosphosignalling & proteolysisdoi:10.1002/j.1460-2075.1995.tb00063.xPMID 7664732
Circuit simulation of genetic networks Science 269:650-656
Proposed modeling genetic networks as circuits, illustrated with the phage lambda lysis-lysogeny decision, treating operons as logic elements with time delays.
landmarkSystems & modellingdoi:10.1126/science.7624793PMID 7624793
Cell cycle regulation and cell type-specific localization of the FtsZ division initiation protein in Caulobacter Proc Natl Acad Sci U S A 93:6314-6319
Showed FtsZ levels vary dramatically over the cell cycle and that after division only the stalked cell contains FtsZ.
Division & shapedoi:10.1073/pnas.93.13.6314PMID 8692812
Cell cycle control by an essential bacterial two-component signal transduction protein Cell 84:83-93
Identified CtrA, an essential response regulator that binds target promoters and controls cell division, stalk synthesis and cell cycle-regulated transcription.
landmarkCell-cycle controlPhosphosignalling & proteolysisdoi:10.1016/s0092-8674(00)80995-2PMID 8548829
Cell-surface display of a Pseudomonas aeruginosa strain K pilin peptide within the paracrystalline S-layer of Caulobacter crescentus Mol Microbiol 26:277-288
Inserted a Pseudomonas pilin peptide at many sites in the S-layer protein RsaA, showing the S-layer can display epitope-size heterologous peptides.
Applicationsdoi:10.1046/j.1365-2958.1997.5711932.xPMID 9383153
Cell type-specific phosphorylation and proteolysis of a transcriptional regulator controls the G1-to-S transition in a bacterial cell cycle Cell 90:415-424
Showed CtrA is controlled by cell type-specific phosphorylation and proteolysis, and that a stable, constitutively active CtrA blocks the G1-to-S transition.
landmarkCell-cycle controlPhosphosignalling & proteolysisdoi:10.1016/s0092-8674(00)80502-4PMID 9267022
Stochastic mechanisms in gene expression Proc Natl Acad Sci U S A 94:814-819
Simulations showed proteins are produced in random bursts from activated promoters, which can partition a cell population into different phenotypes.
Systems & modellingdoi:10.1073/pnas.94.3.814PMID 9023339
Cell cycle-dependent polar localization of chromosome partitioning proteins in Caulobacter crescentus Cell 88:675-684
Showed ParA and ParB localize to both poles of the predivisional cell after replication, with ParB binding DNA near the origin.
landmarkReplication & segregationdoi:10.1016/s0092-8674(00)81910-8PMID 9054507
Protein localization and cell fate in bacteria Science 276:712-718
Review of how protein localization governs replication, segregation, cytokinesis and cell fate, using Caulobacter and Bacillus subtilis as paradigms.
An essential protease involved in bacterial cell-cycle control EMBO J 17:5658-5669
Showed the ClpXP protease is essential for viability and required for cell cycle-dependent CtrA degradation and the G1-to-S transition.
Phosphosignalling & proteolysisCell-cycle controldoi:10.1093/emboj/17.19.5658PMID 9755166
Simulation of prokaryotic genetic circuits Annu Rev Biophys Biomol Struct 27:199-224
Review arguing for molecular-level simulation of bacterial genetic circuits and signaling networks to identify their design principles.
Systems & modellingReviewsdoi:10.1146/annurev.biophys.27.1.199PMID 9646867
Negative control of bacterial DNA replication by a cell cycle regulatory protein that binds at the chromosome origin Proc Natl Acad Sci U S A 95:120-125
Showed CtrA binds five sites in the chromosome replication origin and represses replication in vivo, restricting initiation to the stalked cell.
Cell-cycle controlReplication & segregationdoi:10.1073/pnas.95.1.120PMID 9419339
Cell cycle-dependent polar localization of an essential bacterial histidine kinase that controls DNA replication and cell division Cell 97:111-120
Identified CckA, an essential histidine kinase required for CtrA phosphorylation that localizes to a cell pole in S phase and disperses after division.
landmarkPhosphosignalling & proteolysisCell-cycle controldoi:10.1016/s0092-8674(00)80719-9PMID 10199407
Differential localization of two histidine kinases controlling bacterial cell differentiation Mol Cell 4:683-694
Showed the antagonistic kinases DivJ and PleC localize to opposite poles in a cell cycle-dependent manner, with PleC required for DivJ localization.
Phosphosignalling & proteolysisdoi:10.1016/s1097-2765(00)80379-2PMID 10619016
Global analysis of the genetic network controlling a bacterial cell cycle Science 290:2144-2148
Microarrays of synchronized cells identified 553 cell cycle-regulated genes (19% of the genome), about a quarter under direct or indirect CtrA control.
landmarkCell-cycle controlGenome-wide studiesdoi:10.1126/science.290.5499.2144PMID 11118148
Identification and cell cycle control of a novel pilus system in Caulobacter crescentus EMBO J 19:3223-3234
Identified a pilus gene cluster including pilA and showed late, CtrA-regulated pilA transcription helps time pilus assembly.
Polar developmentdoi:10.1093/emboj/19.13.3223PMID 10880436
Complete genome sequence of Caulobacter crescentus Proc Natl Acad Sci U S A 98:4136-4141
Reported the complete 4,016,942-bp single-chromosome genome (3,767 genes), noting an unusually large set of 105 two-component signaling proteins.
landmarkGenomedoi:10.1073/pnas.061029298PMID 11259647
Genes directly controlled by CtrA, a master regulator of the Caulobacter cell cycle Proc Natl Acad Sci U S A 99:4632-4637
In vivo genomic binding analysis combined with expression data showed CtrA directly regulates at least 95 genes, including 14 regulatory genes.
Cell-cycle controlGenome-wide studiesdoi:10.1073/pnas.062065699PMID 11930012
Generating and exploiting polarity in bacteria Science 298:1942-1946
Review of how dynamic localization of signaling proteins, cytoskeletal proteins and chromosome regions generates and exploits bacterial polarity.
The bacterial cytoskeleton: an intermediate filament-like function in cell shape Cell 115:705-713
Identified crescentin, an intermediate filament-like cytoskeletal protein required for the curved cell shape; without it cells grow as straight rods.
landmarkDivision & shapedoi:10.1016/s0092-8674(03)00935-8PMID 14675535
A bacterial cell-cycle regulatory network operating in time and space Science 301:1874-1877
Perspective arguing that Caulobacter cell-cycle control combines transcriptional circuits with localized, nontranscriptional pathways under a few master regulators.
landmarkCell-cycle controlSystems & modellingReviewsdoi:10.1126/science.1087694PMID 14512618
Oscillating global regulators control the genetic circuit driving a bacterial cell cycle Science 304:983-987
Identified GcrA, a master regulator oscillating out of phase with CtrA, forming a circuit that drives cell-cycle progression and polar morphogenesis.
landmarkCell-cycle controldoi:10.1126/science.1095191PMID 15087506
Cell cycle-dependent dynamic localization of a bacterial response regulator with a novel di-guanylate cyclase output domain Genes Dev 18:715-727
Showed the response regulator PleD has a diguanylate cyclase output domain and is sequestered to the cell pole when activated by phosphorylation.
Phosphosignalling & proteolysisdoi:10.1101/gad.289504PMID 15075296
Cell-cycle progression and the generation of asymmetry in Caulobacter crescentus Nat Rev Microbiol 2:325-337
Review of cell-cycle progression and the generation of asymmetry in Caulobacter crescentus.
Rapid and sequential movement of individual chromosomal loci to specific subcellular locations during bacterial DNA replication Proc Natl Acad Sci U S A 101:9257-9262
Imaged 112 chromosomal loci, showing each has a specific subcellular address in linear order and loci move sequentially there as they are replicated.
Replication & segregationdoi:10.1073/pnas.0402606101PMID 15178755
DnaA coordinates replication initiation and cell cycle transcription in Caulobacter crescentus Mol Microbiol 58:1340-1353
Showed DnaA acts as a global transcription factor, activating genes such as gcrA, ftsZ and podJ and coupling replication initiation to cell-cycle transcription.
Cell-cycle controlReplication & segregationdoi:10.1111/j.1365-2958.2005.04912.xPMID 16313620
Whole-genome transcriptional analysis of heavy metal stresses in Caulobacter crescentus J Bacteriol 187:8437-8449
Transcriptional profiling of chromium, cadmium, selenium and uranium exposure found high uranium tolerance and oxidative-stress protection as the main response.
ApplicationsStress responsesdoi:10.1128/jb.187.24.8437-8449.2005PMID 16321948
Two-component signal transduction pathways regulating growth and cell cycle progression in a bacterium: a system-level analysis PLoS Biol 3:e334
Deletion of 106 two-component genes found 39 required for growth, cell cycle or morphogenesis; phosphotransfer profiling revealed the essential CenK-CenR pathway.
Phosphosignalling & proteolysisdoi:10.1371/journal.pbio.0030334PMID 16176121
Regulation of the bacterial cell cycle by an integrated genetic circuit Nature 444:899-904
Identified ChpT, an essential phosphotransferase in two CckA-initiated phosphorelays that phosphorylate and stabilize CtrA, closing a feedback loop through DivK.
landmarkPhosphosignalling & proteolysisCell-cycle controldoi:10.1038/nature05321PMID 17136100
DnaA couples DNA replication and the expression of two cell cycle master regulators EMBO J 25:346-356
Showed DnaA activates gcrA transcription, linking replication initiation to the DnaA/GcrA/CtrA regulatory cascade that drives cell-cycle progression.
landmarkCell-cycle controlDNA methylationdoi:10.1038/sj.emboj.7600927PMID 16395331
A phospho-signaling pathway controls the localization and activity of a protease complex critical for bacterial cell cycle progression Proc Natl Acad Sci U S A 103:10935-10940
Showed unphosphorylated CpdR localizes ClpXP to the cell pole for CtrA degradation, while CckA-dependent CpdR phosphorylation prevents it.
Phosphosignalling & proteolysisdoi:10.1073/pnas.0604554103PMID 16829582
A landmark protein essential for establishing and perpetuating the polarity of a bacterial cell Cell 124:1011-1023
Identified TipN, a new-pole landmark protein whose relocation at division orients polarity in the progeny; its loss causes pleiotropic polarity defects.
Polar developmentdoi:10.1016/j.cell.2005.12.040PMID 16530047
A dynamically localized protease complex and a polar specificity factor control a cell cycle master regulator Cell 124:535-547
Showed ClpXP is dynamically localized and degrades CtrA at the cell pole, and identified RcdA, required for CtrA polar localization and degradation.
landmarkPhosphosignalling & proteolysisCell-cycle controldoi:10.1016/j.cell.2005.12.033PMID 16469700
MipZ, a spatial regulator coordinating chromosome segregation with cell division in Caulobacter Cell 126:147-162
Identified MipZ, a ParB-associated FtsZ inhibitor whose polar localization restricts Z-ring formation to midcell, coupling segregation to division.
landmarkDivision & shapeReplication & segregationdoi:10.1016/j.cell.2006.05.038PMID 16839883
Adhesion of single bacterial cells in the micronewton range Proc Natl Acad Sci U S A 103:5764-5768
Micromanipulation measured holdfast-mediated adhesion of single cells at 0.11-2.26 micronewtons, reported as the strongest biological adhesive measured.
Polar developmentdoi:10.1073/pnas.0601705103PMID 16585522
A nutrient uptake role for bacterial cell envelope extensions Proc Natl Acad Sci U S A 103:11772-11777
Showed stalks take up and hydrolyze organic phosphate and contain PstS, arguing their long thin shape aids nutrient uptake in dilute environments.
Polar developmentGrowth & physiologydoi:10.1073/pnas.0602047103PMID 16861302
The ECF sigma factor sigma(T) is involved in osmotic and oxidative stress responses in Caulobacter crescentus Mol Microbiol 66:1240-1255
Showed the ECF sigma factor sigma T is needed to survive osmotic and oxidative stress and identified about 40 putative sigma T regulon members.
Stress responsesdoi:10.1111/j.1365-2958.2007.06005.xPMID 17986185
A DNA methylation ratchet governs progression through a bacterial cell cycle Proc Natl Acad Sci U S A 104:17111-17116
Showed promoter methylation state times dnaA and ctrA transcription, so replication-fork passage and late CcrM synthesis act as a ratchet on the cell cycle.
DNA methylationCell-cycle controldoi:10.1073/pnas.0708112104PMID 17942674
Caulobacter crescentus as a whole-cell uranium biosensor Appl Environ Microbiol 73:7615-7621
Engineered a urcA promoter-GFP reporter strain that detects micromolar uranium and distinguished contaminated from uncontaminated groundwater samples.
Applicationsdoi:10.1128/aem.01566-07PMID 17905881
Systems biology of Caulobacter Annu Rev Genet 41:429-441
Review of the genetic network and the phosphosignaling, proteolysis and epigenetic mechanisms that control the Caulobacter cell cycle.
ReviewsSystems & modellingdoi:10.1146/annurev.genet.41.110306.130346PMID 18076330
High-throughput identification of transcription start sites, conserved promoter motifs and predicted regulons Nat Biotechnol 25:584-592
Tiled microarrays identified transcription start sites of 769 genes and 27 promoter motifs, used to predict regulons including stress responses.
landmarkGenome-wide studiesdoi:10.1038/nbt1294PMID 17401361
A comprehensive set of plasmids for vanillate- and xylose-inducible gene expression in Caulobacter crescentus Nucleic Acids Res 35:e137
Characterized a vanillate-inducible promoter and built a set of integrating and replicating vectors for gene expression and protein tagging.
Applicationsdoi:10.1093/nar/gkm818PMID 17959646
A polymeric protein anchors the chromosomal origin/ParB complex at a bacterial cell pole Cell 134:945-955
Identified PopZ, a polymeric polar protein that binds ParB and anchors the segregated chromosome origins at the cell poles.
landmarkReplication & segregationdoi:10.1016/j.cell.2008.07.015PMID 18805088
Characterization of the SOS regulon of Caulobacter crescentus J Bacteriol 190:1209-1218
Identified 37 LexA-dependent genes forming the SOS regulon and confirmed GTTCN7GTTC as the SOS operator.
Stress responsesdoi:10.1128/jb.01419-07PMID 18083815
A self-associating protein critical for chromosome attachment, division, and polar organization in caulobacter Cell 134:956-968
Showed PopZ is a self-associating polar hub that captures ParB-ori complexes and mediates polar localization of CckA and DivJ.
landmarkReplication & segregationDivision & shapedoi:10.1016/j.cell.2008.07.016PMID 18805089
SpoT regulates DnaA stability and initiation of DNA replication in carbon-starved Caulobacter crescentus J Bacteriol 190:6867-6880
Showed carbon starvation raises (p)ppGpp via SpoT, which is required for DnaA proteolysis and for blocking replication initiation in starved swarmer cells.
Stress responsesReplication & segregationdoi:10.1128/jb.00700-08PMID 18723629
A quantitative study of the division cycle of Caulobacter crescentus stalked cells PLoS Comput Biol 4:e9
Built a quantitative model of the stalked-cell cycle based on CtrA, GcrA and DnaA that reproduces wild-type protein time courses and many mutant phenotypes.
Systems & modellingCell-cycle controldoi:10.1371/journal.pcbi.0040009PMID 18225942
Architecture and inherent robustness of a bacterial cell-cycle control system Proc Natl Acad Sci U S A 105:11340-11345
Built a hybrid simulation of cell-cycle control and used formal model checking to show it is robust to stochastic variation and nutrient starvation.
Systems & modellingCell-cycle controldoi:10.1073/pnas.0805258105PMID 18685108
Caulobacter requires a dedicated mechanism to initiate chromosome segregation Proc Natl Acad Sci U S A 105:15435-15440
Showed the parS site is where segregation force is exerted via the essential ParABS system, independent of its position on the chromosome.
Replication & segregationdoi:10.1073/pnas.0807448105PMID 18824683
Second messenger-mediated spatiotemporal control of protein degradation regulates bacterial cell cycle progression Genes Dev 23:93-104
Showed c-di-GMP promotes CtrA degradation through the effector PopA, which localizes to the old pole and recruits CtrA via RcdA.
Phosphosignalling & proteolysisdoi:10.1101/gad.502409PMID 19136627
Why and how bacteria localize proteins Science 326:1225-1228
Review of how and why bacteria deploy proteins to specific subcellular locations in step with the organized deployment of the chromosome.
Quantitative genome-scale analysis of protein localization in an asymmetric bacterium Proc Natl Acad Sci U S A 106:7858-7863
Built a high-throughput fluorescent-fusion imaging pipeline that identified nearly 300 localized proteins, a genome-scale localization map.
Systems & modellingGenome-wide studiesdoi:10.1073/pnas.0901781106PMID 19416866
High-throughput identification of protein localization dependency networks Proc Natl Acad Sci U S A 107:4681-4686
Automated imaging of 854 transposon mutants identified genes affecting localization of PleC, DivJ and CpaE, mapping localization dependency networks.
Systems & modellingGenome-wide studiesdoi:10.1073/pnas.1000846107PMID 20176934
Getting in the loop: regulation of development in Caulobacter crescentus Microbiol Mol Biol Rev 74:13-41
Review of Caulobacter development, emphasizing how multiple processes are integrated and coordinated in space and time.
ReviewsPolar developmentdoi:10.1128/mmbr.00040-09PMID 20197497
A cell-type-specific protein-protein interaction modulates transcriptional activity of a master regulator in Caulobacter crescentus Mol Cell 39:455-467
Identified SciP, a small protein that accumulates in G1 and binds CtrA to inhibit activation of its target genes without affecting CtrA stability or phosphorylation.
Cell-cycle controldoi:10.1016/j.molcel.2010.06.024PMID 20598601
A structural model of anti-anti-σ inhibition by a two-component receiver domain: the PhyR stress response regulator Mol Microbiol 78:290-304
Placed PhyR genetically in the sigma T/NepR general stress pathway and solved its crystal structure, proposing a model of anti-anti-sigma regulation.
Stress responsesdoi:10.1111/j.1365-2958.2010.07323.xPMID 20735776
The genetic basis of laboratory adaptation in Caulobacter crescentus J Bacteriol 192:3678-3688
Whole-genome sequencing mapped the polymorphisms at five loci underlying phenotypic differences between strain CB15 and its lab-adapted derivative NA1000.
A spindle-like apparatus guides bacterial chromosome segregation Nat Cell Biol 12:791-798
Showed ParA forms linear polymers that ParB destabilizes, proposing a burnt-bridge Brownian ratchet for segregation, with TipN keeping it directional.
landmarkReplication & segregationdoi:10.1038/ncb2083PMID 20657594
An essential transcription factor, SciP, enhances robustness of Caulobacter cell cycle regulation Proc Natl Acad Sci U S A 107:18985-18990
Showed SciP is an essential DNA-binding transcription factor forming an incoherent feedforward loop with CtrA that regulates at least 58 genes.
Cell-cycle controldoi:10.1073/pnas.1014395107PMID 20956288
The complex logic of stringent response regulation in Caulobacter crescentus: starvation signalling in an oligotrophic environment Mol Microbiol 80:695-714
Defined the input logic and transcriptional output of the stringent response; the sole Rsh enzyme SpoT binds the ribosome and needs amino-acid starvation to act.
Stress responsesdoi:10.1111/j.1365-2958.2011.07602.xPMID 21338423
The essential genome of a bacterium Mol Syst Biol 7:528
Hyper-saturated Tn-seq defined 1,012 essential genome features at 8-bp resolution, including 480 ORFs, 402 regulatory sequences and 130 non-coding elements.
landmarkGenome-wide studiesdoi:10.1038/msb.2011.58PMID 21878915
Assembly of the Caulobacter cell division machine Mol Microbiol 80:1680-1698
Used synchronized cells to determine the order in which divisome components arrive at the division site, with high temporal resolution.
Division & shapedoi:10.1111/j.1365-2958.2011.07677.xPMID 21542856
The architecture and conservation pattern of whole-cell control circuitry J Mol Biol 409:28-35
Review arguing that the cell-cycle control circuitry is co-conserved across alphaproteobacteria and optimized for robust whole-cell operation.
ReviewsSystems & modellingdoi:10.1016/j.jmb.2011.02.041PMID 21371478
A DNA damage checkpoint in Caulobacter crescentus inhibits cell division through a direct interaction with FtsW Genes Dev 25:1328-1343
Showed DNA damage induces the SOS regulon and inhibits CtrA, and that the SOS-induced protein SidA delays division by binding FtsW.
Stress responsesDivision & shapedoi:10.1101/gad.2038911PMID 21685367
A dynamic complex of signaling proteins uses polar localization to regulate cell-fate asymmetry in Caulobacter crescentus Dev Cell 20:329-341
Showed the unorthodox kinase DivL promotes CckA activity while phosphorylated DivK inhibits CckA by binding DivL, with polar localization protecting CckA.
Phosphosignalling & proteolysisdoi:10.1016/j.devcel.2011.01.007PMID 21397844
Global methylation state at base-pair resolution of the Caulobacter genome throughout the cell cycle Proc Natl Acad Sci U S A 110:E4658-E4667
SMRT sequencing tracked 4,515 GANTC sites through the cell cycle, finding 27 persistently unmethylated sites and 59 candidate epigenetically regulated genes.
DNA methylationGenome-wide studiesdoi:10.1073/pnas.1319315110PMID 24218615
High-resolution mapping of the spatial organization of a bacterial chromosome Science 342:731-734
Used Hi-C to show the chromosome is organized into multiple stable spatial domains, with HU and SMC promoting compaction and arm colinearity.
landmarkReplication & segregationGenome-wide studiesdoi:10.1126/science.1242059PMID 24158908
A constant size extension drives bacterial cell size homeostasis Cell 159:1433-1446
Showed E. coli and C. crescentus maintain size homeostasis by adding a constant size between divisions rather than dividing at a critical size.
Growth & physiologydoi:10.1016/j.cell.2014.11.022PMID 25480302
Cell fate regulation governed by a repurposed bacterial histidine kinase PLoS Biol 12:e1001979
Crystal structure and biochemistry showed the pseudokinase DivL preferentially binds phosphorylated DivK, repurposing kinase domains as a regulator sensor.
Phosphosignalling & proteolysisdoi:10.1371/journal.pbio.1001979PMID 25349992
Cell cycle transition from S-phase to G1 in Caulobacter is mediated by ancestral virulence regulators Nat Commun 5:4081
Identified MucR1/2, zinc-finger regulators related to alphaproteobacterial virulence regulators, as part of a module directing the S-to-G1 transcriptional switch.
Cell-cycle controldoi:10.1038/ncomms5081PMID 24939058
Scaling laws governing stochastic growth and division of single bacterial cells Proc Natl Acad Sci U S A 111:15912-15917
Single-cell measurements showed exponential growth and division at a critical size multiple (about 1.8), with distributions collapsing onto universal curves.
Growth & physiologydoi:10.1073/pnas.1403232111PMID 25349411
The coding and noncoding architecture of the Caulobacter crescentus genome PLoS Genet 10:e1004463
Combined RNA-seq, 5'-RACE, ribosome profiling and proteomics to map transcripts and coding regions; most start codons lack Shine-Dalgarno sites.
Genome-wide studiesdoi:10.1371/journal.pgen.1004463PMID 25078267
Cyclic di-GMP acts as a cell cycle oscillator to drive chromosome replication Nature 523:236-239
Showed c-di-GMP levels oscillate in the cell cycle and that c-di-GMP binds CckA, switching it from kinase to phosphatase to allow replication initiation.
landmarkCell-cycle controlPhosphosignalling & proteolysisdoi:10.1038/nature14473PMID 25945741
The global regulatory architecture of transcription during the Caulobacter cell cycle PLoS Genet 11:e1004831
Mapped 2,726 transcription start sites through the cell cycle, finding 586 cell cycle-regulated and 529 binding sites for the master regulators.
Cell-cycle controlGenome-wide studiesdoi:10.1371/journal.pgen.1004831PMID 25569173
Cell cycle control in Alphaproteobacteria Curr Opin Microbiol 30:107-113
Review of conserved cell-cycle regulators and signals such as c-di-GMP and (p)ppGpp in Alphaproteobacteria, building on Caulobacter work.
CauloBrowser: A systems biology resource for Caulobacter crescentus Nucleic Acids Res 44:D640-D645
Introduced CauloBrowser, an online resource integrating expression, essentiality, localization and methylation datasets for Caulobacter.
Genome-wide studiesdoi:10.1093/nar/gkv1050PMID 26476443
Obstruction of pilus retraction stimulates bacterial surface sensing Science 358:535-538
Showed pili stop retracting upon surface contact and that obstructing retraction alone triggers holdfast synthesis, revealing a surface-sensing mechanism.
Polar developmentdoi:10.1126/science.aan5706PMID 29074778
Selective sequestration of signalling proteins in a membraneless organelle reinforces the spatial regulation of asymmetry in Caulobacter crescentus Nat Microbiol 5:418-429
Showed the PopZ polar microdomain selectively concentrates CckA, ChpT and CtrA and enhances phosphosignaling, producing a gradient of active CtrA.
Phosphosignalling & proteolysisdoi:10.1038/s41564-019-0647-7PMID 31959967
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